synergistic effect of lps, ifn- and iron on apoptosis of balb/c mice macrophages following nitric oxide production

Authors

leila pirdel college of medicine and medical sciences, islamic azad university, ardebil branch, ardebil, iran

ahmad zavaran hosseini department of immunology, school of medicine, tarbiat modares university, tehran, iran

abstract

objective(s) previous studies have demonstrated that the nitric oxide (no) dependent death of murine peritoneal macrophages activated in vitro with ifn-g and lps is mediated through apoptosis. in the present study, we investigated the synergistic effect of lps, ifn-g and iron on no production and apoptosis. materials and methods after determination of iron cytotoxicity, the peritoneal macrophages of balb/c mice were cultured with iron, lps, and ifn-g separately, or a mixture of these for 18 hr at 37 ◦c. then after 18 hr incubation, the level of no in supernatant was measured by the griess method. at the same time, after incubation with ethidium bromide and acridine orange dye, the apoptotic macrophages were detected by fluorescence microscopy. results no production was significantly greater than the control group in macrophages exposed to iron,lps, or ifn-g  alone (p=0.02), while no significant difference was detected in apoptosis rate in the presence of lps (p=0.08). however, the differences were remarkable between no production and apoptosis rate in the presence of iron, lps and ifn-g (p≤0.05). conclusion these findings indicate the immunostimulatory effect of iron on no production by ifn-g and lps.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Synergistic Effect of LPS, IFN- and Iron on Apoptosis of Balb/c Mice Macrophages Following Nitric Oxide Production

Objective(s) Previous studies have demonstrated that the nitric oxide (NO) dependent death of murine peritoneal macrophages activated in vitro with IFN-g and LPS is mediated through apoptosis. In the present study, we investigated the synergistic effect of LPS, IFN-g and iron on NO production and apoptosis. Materials and Methods After determination of iron cytotoxicity, the peritoneal macrop...

full text

Effect of Neutrophils on Nitric Oxide Production from Stimulated Macrophages

Background: During the initial phase of an infection, there is an upregulation of inducible nitric oxide synthase in the macrophages for the production of nitric oxide. This is followed by the recruitment of polymorphonuclear leukocytes (neutrophils) which release arginase. Arginase competes with inducible nitric oxide synthase for a common substrate L-arginine. Objective: To investigate whethe...

full text

effect of neutrophils on nitric oxide production from stimulated macrophages

background: during the initial phase of an infection, there is an upregulation of inducible nitric oxide synthase in the macrophages for the production of nitric oxide. this is followed by the recruitment of polymorphonuclear leukocytes (neutrophils) which release arginase. arginase competes with inducible nitric oxide synthase for a common substrate l-arginine. objective: to investigate whethe...

full text

EFFECT OF FIVE ALPHA DIHYDROTESTOSTERONE (5α-DHT) ON CYTOKINE PRODUCTION BY PERITONEAL MACROPHAGES OF NZB/BALBc MICE

One of the mechanisms involved in the regulation of the immune system by steroid hormones could be the monocytic-macrophage system. In this study the effect of the male hormone 5a-DHT on cytokine release by peritoneal macrophages (mΦ) of male and female NZB/BALBc mice was investigated. Macrophages from male mice activated with LPS produced a greater amount of IL-1β (21.8%) (p<0.05) and IL-...

full text

effect of seed priming and irrigation regimes on yield,yield components and quality of safflowers cultivars

این مطالعه در سال 1386-87 در آزمایشگاه و مزرعه پژوهشی دانشگاه صنعتی اصفهان به منظور تعیین مناسب ترین تیمار بذری و ارزیابی اثر پرایمینگ بر روی سه رقم گلرنگ تحت سه رژیم آبیاری انجام گرفت. برخی از مطالعات اثرات سودمند پرایمینگ بذر را بر روی گیاهان مختلف بررسی کرده اند اما در حال حاضر اطلاعات کمی در مورد خصوصیات مربوط به جوانه زنی، مراحل نموی، عملکرد و خصوصیات کمی و کیفی بذور تیمار شده ژنوتیپ های م...

My Resources

Save resource for easier access later


Journal title:
iranian journal of basic medical sciences

جلد ۱۱، شماره ۳، صفحات ۱۹۰-۱۹۶

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023